Food preservation | Definition, Importance, & Methods (2024)

Bacteria

Bacteria are unicellular organisms that have a simple internal structure compared with the cells of other organisms. The increase in the number of bacteria in a population is commonly referred to as bacterial growth by microbiologists. This growth is the result of the division of one bacterial cell into two identical bacterial cells, a process called binary fission. Under optimal growth conditions, a bacterial cell may divide approximately every 20 minutes. Thus, a single cell can produce almost 70 billion cells in 12 hours. The factors that influence the growth of bacteria include nutrient availability, moisture, pH, oxygen levels, and the presence or absence of inhibiting substances (e.g., antibiotics).

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The nutritional requirements of most bacteria are chemical elements such as carbon, hydrogen, oxygen, nitrogen, phosphorus, sulfur, magnesium, potassium, sodium, calcium, and iron. The bacteria obtain these elements by utilizing gases in the atmosphere and by metabolizing certain food constituents such as carbohydrates and proteins.

Temperature and pH play a significant role in controlling the growth rates of bacteria. Bacteria may be classified into three groups based on their temperature requirement for optimal growth: thermophiles (55–75 °C, or 130–170 °F), mesophiles (20–45 °C, or 70–115 °F), or psychrotrophs (10–20 °C, or 50–70 °F). In addition, most bacteria grow best in a neutral environment (pH equal to 7).

Bacteria also require a certain amount of available water for their growth. The availability of water is expressed as water activity and is defined by the ratio of the vapour pressure of water in the food to the vapour pressure of pure water at a specific temperature. Therefore, the water activity of any food product is always a value between 0 and 1, with 0 representing an absence of water and 1 representing pure water. Most bacteria do not grow in foods with a water activity below 0.91, although some halophilic bacteria (those able to tolerate high salt concentrations) can grow in foods with a water activity lower than 0.75. Growth may be controlled by lowering the water activity—either by adding solutes such as sugar, glycerol, and salt or by removing water through dehydration.

The oxygen requirements for optimal growth vary considerably for different bacteria. Some bacteria require the presence of free oxygen for growth and are called obligate aerobes, whereas other bacteria are poisoned by the presence of oxygen and are called obligate anaerobes. Facultative anaerobes are bacteria that can grow in both the presence or absence of oxygen. In addition to oxygen concentration, the oxygen reduction potential of the growth medium influences bacterial growth. The oxygen reduction potential is a relative measure of the oxidizing or reducing capacity of the growth medium.

When bacteria contaminate a food substrate, it takes some time before they start growing. This lag phase is the period when the bacteria are adjusting to the environment. Following the lag phase is the log phase, in which population grows in a logarithmic fashion. As the population grows, the bacteria consume available nutrients and produce waste products. When the nutrient supply is depleted, the growth rate enters a stationary phase in which the number of viable bacteria cells remains the same. During the stationary phase, the rate of bacterial cell growth is equal to the rate of bacterial cell death. When the rate of cell death becomes greater than the rate of cell growth, the population enters the decline phase.

A bacterial population is expressed either per gram or per square centimetre of surface area. Rarely does the total bacterial population exceed 10¹⁰ cells per gram. A population of less than 10⁶ cells per gram does not cause any noticeable spoilage except in raw milk. Populations of between 10⁶ and 10⁷ cells per gram cause spoilage in some foods; for example, they can generate off-odours in vacuum-packaged meats. Populations of between 10⁷ and 10⁸ cells per gram produce off-odours in meats and some vegetables. At levels above 5 × 10⁷ cells per gram, most foods exhibit some form of spoilage.

When the conditions for bacterial cell growth are unfavourable (e.g., low or high temperatures or low moisture content), several species of bacteria can produce resistant cells called endospores. Endospores are highly resistant to heat, chemicals, desiccation (drying out), and ultraviolet light. The endospores may remain dormant for long periods of time. When conditions become favourable for growth (e.g., thawing of meats), the endospores germinate and produce viable cells that can begin exponential growth.